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DNA Topoisomerase Protocols -Volume 1

	Author(s):	Osheroff N., Bjornsti M.A.
	Collection:	Methods in Molecular Biology 094
	Publisher:
	Year:	1999
	Language:	English
	Pages:	317 pages
	Size:	3.87 MB
	Extension:	PDF

[tab] [content title="Description"]DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems. [/content] [content title="Content"] [/content] [content title="About the author"]Neil Osheroff, Ph.D. Professor of Biochemistry Professor of Medicine (Hematology/Oncology) John G. Coniglio Chair in Biochemistry Past-Director, Academy for Excellence in Education Co-Leader, Foundations of Medical Knowledge Phase.Mary-Ann Bjornsti, Ph.D., is Chair of the Department of Pharmacology and Toxicology at UAB, and holds the Newman H. Waters Chair of Clinical Pharmacology. She also serves as co-Leader of the Cancer Cell Biology Program and Associate Director for Translational Research in the UAB Comprehensive Cancer Center. She received her Ph.D. from the University of Minnesota in Genetics, followed by a Fogarty Fellowship at the Biozentrum in Basel Switzerland, and post-doctoral training at Harvard University with Dr. James C. Wang. [/content] [/tab]

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